Flow-through method to separate protein monomers from aggregates

Request Number N558213
Author GSK
Need details

Seeking scalable flow-through methods to separate monomers from aggregates


The use of proteins as drugs is an important, and growing, sector in the pharmaceutical market. Antibodies, proteins and peptides are used. They are expressed in mammalian cells, grown in large fermenters containing 1000L (or more) of culture media. The target protein is secreted into solution and must be purified.


Proteins can aggregate during production. The aggregated protein must be removed from solution. Aggregation reduces the biological activity of proteins and it is critically important that biopharmaceuticals are highly active.


We are seeking scalable flow-through methods to separate monomers from aggregates. We are looking at continuous media manufacturing and this technology will help provide additional sterility assurance. We are keen identify partners who can sell, supply or collaborate with us to develop appropriate technology.


Key Success Criteria

• Yields of monomer > 70%

• Overall purity of product is maintained

• Works with proteins from approximately 25kDa to 160kDa


Possible Approaches

GSK is open to all approaches capable of achieving the criteria set forth above.



Approaches not of interest

Bind and elute method.



Preferred Collaboration types
  • Joint Development
  • Contract Research
  • Supply Agreement
Items to be submitted

We are looking for concise, non-confidential proposals. The proposal should:

• Describe the technical approach

• Include a description of the responding organisation or team and their experience


Please use the Respond button in order to submit your proposal.


We recommend:

• including a concise abstract summarising your submission

• attaching your proposal

• attaching any additional documents


Please note that only non-confidential information can be accepted. By submitting a response you confirm that the response does not contain any confidential information.


Point of Contact
Stephen Clulow
Area of Interest
  • Aerospace & Defense
  • Chemical Manufacturing
  • Commercial & Industrial Design
  • Family & Consumer Products & Services
  • Food & Beverage
  • Manufacturing & Fabrication
  • Materials Processing
  • Medical Products & Services
  • Pharmaceuticals
  • Research Organizations
  • Utilities
Due Date
April 21, 2017
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