Request for Proposal
Status: RFP is Closed

Technology of in vivo Fluorescence Labeling of Any Protein or mRNA Using Antibodies/Probes

Request Number
Due Date
Mar 27
Program Manager

Joint/contract research, joint/contract development, licensing


Selection of a potential partner: Within 6 months
Initiation of joint research: After 1 year


Budget for this project: Budget for joint development has been secured.
(To be discussed depending on the details of proposal)


How to  Apply
Click on "Respond", complete the form to the extent possible, and submit along with other attachment files available. 
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RFP was closed on
Apr 2019

RFP Title


Technology of in  vivo Fluorescence Labeling of Any Protein or mRNA Using Antibodies/Probes
RFP Description

NineSigma, representing a major food manufacturer, seeks a technology of in vivo fluorescence labeling of any protein or mRNA using antibodies/probes. Specifically, the client anticipates proposals for technologies such as:

  • Technology to design/produce fluorescence-labeled detection probes (antibodies, low molecular weight probes, etc.)
  • Technology to easily introduce the fluorescence-labeled detection probe into living cells in a highly efficient manner
Key Success Criteria

Requirements for the Anticipated Technology

The client seeks a technology that meets (1) and (2) described below. It is desirable that the technology meets both (1) and (2); however, proposals for a technology that meets at least one of them are also welcome to participate.

    (1)Technology to design/produce detection probes

  • Should be able to design/produce probes that enable in vivo fluorescence labeling of any protein or mRNA specifically (It is anticipated that re-designing/producing will be conducted for each target protein or mRNA.)
  • Should have fluorescence intensity that can be measured using a flow cytometer after fluorescence labeling 

    (2)Technology to easily introduce the detection probe into living cells

  • Should be able to easily introduce the detection probe into living cells (e.g. permeation)
  • Efficiency of introduction: For the cell group, desirably 50% or more; at least 10%
  • It is desirable that the amount of protein and mRNA in living cells remains as much as possible the same at the time of introduction in cells.

The client currently seeks a technology applicable to yeasts and filamentous fungi, particularly. However, proposals for a technology that will likely be applied to living cells of various species, such as animal cells and plant cells, are desired.

Possible Approaches

Anticipated Approaches

Anticipated approaches include, but are not limited to, the following:

  • Technology to design/produce fluorescence-labeled low molecular weight antibodies that can be easily introduced into living cells by permeation, etc.
  • Technology to design/produce low molecular weight fluorescence probes that can be easily introduced into living cells by permeation, etc. and can specifically detect any target substance
  • Technology using protein nanocarriers (cationic lipids, membrane-permeable peptides, etc.) that can introduce fluorescence-labeled antibodies into living cells
Approaches not of Interest

Approaches Not of Interest

The following approaches are not of interest:

  • Proposals that are targeted only at specific protein or mRNA in living cells
  • Proposals that require an advanced technology, such as microinjection
  • Proposals that require genetic recombination of target cells
    • Fusion of target protein and fluorescence protein, expression of antibodies in living cells, etc.
  • Proposals using an approach involving a high degree of cell death
  • Proposals for a technology in which the detection probe is introduced into only a part of the cell group
Items to be Submitted


The client aims at rapid collection of living cells with specific characteristics using the fluorescence activated cell sorter (FACS) by developing an easy in vivo fluorescence labeling technology for any protein or mRNA. While many fluorescence labeling technologies targeting a specific substance in living cells already exist, it is still challenging to develop a versatile technology that can be applied to any target protein or mRNA.


Notes on Response

Responses should include the details of proposal in a concise manner. Please attach supplemental files as needed.

Note that responses should not include any confidential information.


Response evaluation

The client will evaluate all responses with the following criteria.

  • Overall scientific and technical merit
  • Approach to proof of concept or performance
  • Economic potential of concept
  • Realism of the proposed plan (action items, timeline, roles, deliverables, cost estimation)
  • Potential for proprietary position
  • Respondents’ capability and related experiences


Anticipated Project Process

After reviewing submitted proposals, the client possibly ask clarifying questions before selecting the most suitable candidates for collaboration. The client will select best candidates through evaluations. During the selection process, the client may execute NDA with selected respondents, seek further information disclosure, and discuss specific development targets or potential opportunities.

The client will execute necessary agreements with the selected respondents and move to the advanced development phase. Specifics of any collaboration will be determined through consultation with the concerned parties.

Preferred Collaboration Types
Area of Interest