Production of useful substances, such as proteins for pharmaceutical and food applications using bacteria and other microorganisms, are widely conducted. As the production volume of these substances is affected by culture conditions, strict pH control is crucial for stable production.
While sensors and probes used to measure pH changes in entire batches of cultures are already commercially available, it is difficult to understand the state of individual bacterial cells accurately. In the meantime, R&D activities to develop a pH measurement technology for individual bacterial cells by using fluorescent protein as a reporter are widely conducted, and the target includes not only bacteria, but also mammalian cells and yeasts.
Therefore, the client has issued this open request to seek proposals from organizations around the world in order to accelerate the R&D of this technology.