Rapid and High-Sensitivity Measurement of Bacterial Concentration

Request Number REQ6366552
Due Date March 7, 2018
Request for Proposal Details
RFP Title
Rapid and High-Sensitivity Measurement of Bacterial Concentration
RFP Description

NineSigma, representing a multibillion dollar manufacturer, seeks technology for rapid and high-sensitivity measurement of bacterial concentration in sample. The client particularly anticipates an electrical way to measure bacterial concentration employing dielectrophoretic impedance method, however a wide variety of proposals are also welcome.


The leading manufacturer, a client of NineSigma, is seeking to realize a highly sensitive measurement device of bacteria concentration. In the medical and healthcare fields, market demand on such rapid bacteria measurement system will presumably increase, thanks to growing interest in the Point Of Care (POC) applications.


The client has continuously made enormous efforts for technology development in years. However it has decided to carry out this open request to establish a partnerships with an external party that pursues potential technologies, for reaching its development goals within a shorter period of time.


Key Success Criteria

Final requirements to be ultimately achieved

  • Type of bacteria to be detected: Not specified, but a technology should be capable to measure the bacterial concentration of even a sample containing numerous types of bacteria, such as oral microbe.
  • Lowest concentration limit to be measured: 102 – 103 cfu/mL
  • Measurement time: Within 2 hours
  • Minimum sample volume to be measured: Approx. 0.1 mL; process such as mixing a tiny amount of original sample into several mL of purified water using a cotton swab to increase the sample volume is allowed
  • Pretreatment such as concentrating/culturing bacteria: Preferred to be completely unnecessary or reasonably simple
  • Device size: Desirably to be desktop or more compact, and portable.


Since the above goals are quite challenging, technology proposal capable to measure the bacteria concentration of approx. 105 cfu/mL at this moment is highly welcomed. The proposed technology hopefully has a potential to satisfy the final requirements through an additional development of two years.


Possible Approaches

Possible approaches include, but not limited to, the followings or any combination of them. Electrical measurement methods are highly anticipated.

  • Electrical measurement technology, e.g., dielectrophoretic impedance method
  • Optical measurement technology, e.g., Raman spectroscopy
  • Biological measurement technology, e.g., immunocapture assay


Anticipated Project Phases or Project Plan

Respondents should submit proposals using the attached Response Template.


The client will review submitted proposals and possibly ask clarifying questions before selecting the most suitable candidates for collaboration. The client will select the best candidate(s) through evaluations. During the selection process, the client may execute non-disclosure agreements (NDA) with selected respondent(s), seek further information disclosure, and discuss specific development targets or potential opportunities.

The client will execute necessary agreement(s) with the selected respondent(s) and move to the advanced development phase. Specifics of any collaboration will be determined through consultation with the concerned parties.


Preferred Collaboration types
  • Joint Development
  • Contract Research
  • Technology Licensing
  • Supply Agreement
Items to be submitted

Responses will use the Proposal Template which is linked to the here and include the following items:

  • Overview of the technology
  • Unique point of the technology
  • Development stage (Concept stage / technology verification / implementation into a device)
  • Current performance
    • Type of measurable bacteria
    • Lower concentration limit of measurement
    • Measurement time
    • Minimum sample volume for measurement
    • Measurement accuracy
    • Necessary pretreatment process (if any)
    • Anticipated device size
  • Current challenges and future plans to satisfy the final requirements


Area of Interest
Request Priority