A wide range of technologies are anticipated from companies or academic institutions possessing the following liquid-phase (solution-phase) synthesis technologies.
Anticipated liquid-phase synthesis technologies:
Technologies for synthesizing in a fully liquid phase
- Technologies capable of synthesizing oligonucleotides in a liquid (solution) phase, without loading nucleosides on a solid phase carrier (support) or the like (including solvent-soluble carriers).
- Technologies capable of synthesizing oligonucleotides through elongation not only by monomer units, but also elongation by short-chain oligomers, such as dimers and trimers. (i.e. “block synthesis”)
- It is desirable that manufacturing lead time can be decreasing through the avoiding purification processes, the shortening of processes, or the using concise processes.
- Liquid-phase synthesis technologies using phase switching
- Oligonucleotide synthesis technologies using a soluble carrier that disperses/ precipitates according to the solvent used.
- In addition to the above, technologies using ionic liquids, fluorous solvents, etc. are also acceptable.
Anticipated component technologies
Proposals for technologies which fulfill any one of the following are anticipated.
- Technologies with efficient phosphodiester bond formation reactions
- Should be more cost-effective than the conventional amidite method
- Superior reactivity
- Intermediate shows good properties, such as stability.
- Technologies with phase switching soluble carriers capable of imparting favorable physical properties to oligonucleotides
- Capable of imparting solubility and the like needed for oligonucleotide synthesis reactions
- High precision analysis is feasible
- Efficient purification is feasible
- Feasible in “block synthesis”.
Without restricting proposals to the above, proposals for any innovative technology which greatly reduces the costs of oligonucleotide synthesis are welcome.
Targeted types of nucleic acids
- Modified nucleotides such as 2’ -OMe, 2’ -F, LNA (Locked Nucleic Acid), BNA (Bridged Nucleic Acid)
- Phosphorothioate linkages